Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells.
Identifieur interne : 004F93 ( Main/Exploration ); précédent : 004F92; suivant : 004F94Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells.
Auteurs : Tetsuya Mizutani [Japon] ; Shuetsu Fukushi ; Masaaki Murakami ; Toshio Hirano ; Masayuki Saijo ; Ichiro Kurane ; Shigeru MorikawaSource :
- FEBS letters [ 0014-5793 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : DNA-Binding Proteins, Mitogen-Activated Protein Kinases, Trans-Activators, Tyrosine.
- metabolism : Cell Nucleus, SARS Virus.
- Animals, Chlorocebus aethiops, Phosphorylation, STAT3 Transcription Factor, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) has become a global public health emergency. p38 mitogen-activated protein kinase (MAPK) and its downstream targets are activated in SARS coronavirus (SARS-CoV)-infected Vero E6 cells and activation of p38 MAPK enhances the cytopathic effects of SARS-CoV infection. In addition, weak activation of Akt cannot prevent SARS-CoV infection-induced apoptosis in Vero E6 cells. In the present study, we demonstrated that signal transducer and activator of transcription (STAT) 3, which is constitutively phosphorylated at tyrosine (Tyr)-705 and slightly phosphorylated at serine (Ser)-727 in Vero E6 cells, was dephosphorylated at Tyr-705 on SARS-CoV infection. In addition to phosphorylation of p38 MAPK in virus-infected cells, other MAPKs, i.e., extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK), were phosphorylated. Although inhibitors of ERK1/2 and JNK (PD98059 and SP600125) had no effect on phosphorylation status of STAT3, inhibitors of p38 MAPK (SB203580 and SB202190) partially inhibited dephosphorylation of STAT3 at Tyr-705. Tyr-705-phosphorylated STAT3 was localized mainly in the nucleus in mock infected cells, whereas STAT3 disappeared from the nucleus in virus-infected cells. As STAT3 acts as an activator of transcription in the nucleus, these results suggest that STAT3 lacks its activity on transcription in SARS-CoV-infected Vero E6 cells.
DOI: 10.1016/j.febslet.2004.10.005
PubMed: 15527783
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002A62
- to stream PubMed, to step Curation: 002A62
- to stream PubMed, to step Checkpoint: 002962
- to stream Ncbi, to step Merge: 000B90
- to stream Ncbi, to step Curation: 000B90
- to stream Ncbi, to step Checkpoint: 000B90
- to stream Main, to step Merge: 005352
- to stream Main, to step Curation: 004F93
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells.</title><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan. tmizutan@nih.go.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></author><author><name sortKey="Murakami, Masaaki" sort="Murakami, Masaaki" uniqKey="Murakami M" first="Masaaki" last="Murakami">Masaaki Murakami</name></author><author><name sortKey="Hirano, Toshio" sort="Hirano, Toshio" uniqKey="Hirano T" first="Toshio" last="Hirano">Toshio Hirano</name></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15527783</idno><idno type="pmid">15527783</idno><idno type="doi">10.1016/j.febslet.2004.10.005</idno><idno type="wicri:Area/PubMed/Corpus">002A62</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A62</idno><idno type="wicri:Area/PubMed/Curation">002A62</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A62</idno><idno type="wicri:Area/PubMed/Checkpoint">002962</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002962</idno><idno type="wicri:Area/Ncbi/Merge">000B90</idno><idno type="wicri:Area/Ncbi/Curation">000B90</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B90</idno><idno type="wicri:doubleKey">0014-5793:2004:Mizutani T:tyrosine:dephosphorylation:of</idno><idno type="wicri:Area/Main/Merge">005352</idno><idno type="wicri:Area/Main/Curation">004F93</idno><idno type="wicri:Area/Main/Exploration">004F93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells.</title><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan. tmizutan@nih.go.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></author><author><name sortKey="Murakami, Masaaki" sort="Murakami, Masaaki" uniqKey="Murakami M" first="Masaaki" last="Murakami">Masaaki Murakami</name></author><author><name sortKey="Hirano, Toshio" sort="Hirano, Toshio" uniqKey="Hirano T" first="Toshio" last="Hirano">Toshio Hirano</name></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Nucleus (metabolism)</term><term>Chlorocebus aethiops</term><term>DNA-Binding Proteins (metabolism)</term><term>Mitogen-Activated Protein Kinases (metabolism)</term><term>Phosphorylation</term><term>SARS Virus (metabolism)</term><term>STAT3 Transcription Factor</term><term>Trans-Activators (metabolism)</term><term>Tyrosine (metabolism)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Facteur de transcription STAT-3</term><term>Mitogen-Activated Protein Kinases (métabolisme)</term><term>Noyau de la cellule (métabolisme)</term><term>Phosphorylation</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Transactivateurs (métabolisme)</term><term>Tyrosine (métabolisme)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Binding Proteins</term><term>Mitogen-Activated Protein Kinases</term><term>Trans-Activators</term><term>Tyrosine</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term><term>Noyau de la cellule</term><term>Protéines de liaison à l'ADN</term><term>Transactivateurs</term><term>Tyrosine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Phosphorylation</term><term>STAT3 Transcription Factor</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Facteur de transcription STAT-3</term><term>Phosphorylation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) has become a global public health emergency. p38 mitogen-activated protein kinase (MAPK) and its downstream targets are activated in SARS coronavirus (SARS-CoV)-infected Vero E6 cells and activation of p38 MAPK enhances the cytopathic effects of SARS-CoV infection. In addition, weak activation of Akt cannot prevent SARS-CoV infection-induced apoptosis in Vero E6 cells. In the present study, we demonstrated that signal transducer and activator of transcription (STAT) 3, which is constitutively phosphorylated at tyrosine (Tyr)-705 and slightly phosphorylated at serine (Ser)-727 in Vero E6 cells, was dephosphorylated at Tyr-705 on SARS-CoV infection. In addition to phosphorylation of p38 MAPK in virus-infected cells, other MAPKs, i.e., extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK), were phosphorylated. Although inhibitors of ERK1/2 and JNK (PD98059 and SP600125) had no effect on phosphorylation status of STAT3, inhibitors of p38 MAPK (SB203580 and SB202190) partially inhibited dephosphorylation of STAT3 at Tyr-705. Tyr-705-phosphorylated STAT3 was localized mainly in the nucleus in mock infected cells, whereas STAT3 disappeared from the nucleus in virus-infected cells. As STAT3 acts as an activator of transcription in the nucleus, these results suggest that STAT3 lacks its activity on transcription in SARS-CoV-infected Vero E6 cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name><name sortKey="Hirano, Toshio" sort="Hirano, Toshio" uniqKey="Hirano T" first="Toshio" last="Hirano">Toshio Hirano</name><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Murakami, Masaaki" sort="Murakami, Masaaki" uniqKey="Murakami M" first="Masaaki" last="Murakami">Masaaki Murakami</name><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004F93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004F93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15527783
|texte= Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15527783" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |